Abstract:  A wound is disruption of normal skin integrity caused by physical, chemical and biological factors. Wound healing is a complex biological process involving tissue repair and regeneration. Traditional wound dressings provide basic protection but often lack bioactive properties that actively promote healing. Medicinal plants have been widely used for their therapeutic potential including wound healing. Calotropis gigantea and Carica papaya are well known for their bioactive compounds. Which contribute to accelerated wound healing. This study focuses on the comparative evaluation of the wound healing properties of Calotropis gigantea and Carica papaya latex and their incorporation into bioactive wound healing gauze. Latex from both plants was collected and analyzed for phytochemical composition, antioxidant properties, anti-inflammatory effects and antimicrobial activity. Characterization techniques such as UV-Visible spectroscopy and FTIR analysis were used to identify functional groups responsible for their bioactivity. The latex was then incorporated into a gauze dressing and tested for antimicrobial effectiveness against wound pathogens. The resulting wound healing gauze provides a safer and more effective approach to wound management. Comparative analysis of Calotropis gigantea and Carica papaya latex extracts are both possess significant wound healing properties, with differences in antioxidant, anti-inflammatory, and antimicrobial activities. The findings indicate that both Calotropis gigantea and Carica papaya latex significantly contribute to wound healing by enhancing tissue regeneration , Reducing microbial infections and accelerating wound contraction. The development of latex infused wound dressings provides a natural, cost effective alternative to conventional wound care products.