25, April 2025

Mammosphere Formation from Breast Cancer MDA-MB-468 cell line

VOLUME-9; ISSUE-4; APR-2025

Author(s): Tawari Erebi Patricia

Authors Affiliations:

Department of Chemical Pathology, Faculty of Basic Clinical Sciences, College of Health Science, Niger Delta University, Bayelsa State, Nigeria

DOIs:10.2017/IJRCS/202504018     |     Paper ID: IJRCS202504018


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Abstract:    A distinctive way to enhance breast cancer stem cells (BCSCs) is to cultivate cells in an environment that is non-adherent and non-differentiating.  The mammosphere formation assay is a useful technique for assessing the potential BCSCs in non-adherent in vitro cultures.  This work aimed to generate mammosphere from MDA-MB-468 breast cancer (BC) cell lines, useful as prototypes to research many aspects of BCSC behaviour.  According to the study's data, the generated mammosphere expressed CD44+/CD24−/low and had a high mammosphere formation efficiency (MFE), suggesting the potential for tumour formation.  Mammospheres made from BC cell lines can therefore be used as suitable models to explain the function of BCSCs

     
Key Words:  Mammosphere formation, Mammosphere-forming efficiency, CD44+/CD24−/low, MDA-MB-468 cell line, Breast cancer stem cells (BCSCs).  

Tawari Erebi Patricia(2025); Mammosphere Formation from Breast Cancer MDA-MB-468 cell line, International Journal of Research Culture Society,    ISSN(O): 2456-6683,  Volume – 9,   Issue –  4,  Pp. 98-103.      Available on – https://ijrcs.org/

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